William G

William G. Ross, MA

3945 Deep Woods Road,

Earlysville, VA 22936

Office 434 982-3813

Wgr8w@virginia.edu

Education

Bachelor of Arts: 1983
State University of New York at Geneseo
Program: Biology

Master of Arts: 1985
State University of New York at Geneseo
Program: Biology/Immunology

Professional Experience

1985-1987

Research Scientist, Flow Cytometry / Microbiologist,
NIH/NCI, Surgery Branch.
Bethesda, MD

1987-1988

Instrument Development / Application Support
Becton Dickinson Immunocytometry Systems
San Jose, CA

1988-1991

Research Associate,
Clinical Research Laboratory/AIDS Research Laboratory
Stanford University Hospital/Stanford University Genetics
Stanford, CA

1991-1993

Research Associate/Consultant
Clinical /Research Flow Cytometry Laboratories
School of Medicine, East Carolina University.
Greenville, NC

1993-2001

Research Scientist / Director University FACS Core,
Department of Medical Education, University of Virginia.
Charlottesville, VA

2001- present

Research Instructor in Medicine
Digestive Health Research Center Immunology Core
Department of Medicine
University of Virginia
Charlottesville, VA

Publications

J. Skibber, M.T. Lotze, I. Uppenkamp, W. Ross, S.A. Rosenberg. Identification and Expansion of Human Lymphokine-Activated Killer Cells: Implications for the Immunotherapy of Cancer.
Journal of Surgical Research (1987), 42: 613-621.

J. Skibber, M.T. Lotze, L. Muul, I. Uppenkamp, W. Ross, S. A. Rosenberg. Human Lymphokine-Activated Killer Cells: Further Isolation and Characterization of the Precursor and Effector Cell.
Natural Immunity and Cell Growth Regulation (1987), 6: 291-305.

F. Staal, M. Roederer, D. Israelski, J. Bubp, L.A. Mole, D. McShane, C. Deresinski, W. Ross, H. Sussman, P.A. Raju, M. Walker, S. Ela, W.A. More, L. Herzenberg, L.A. Herzenberg. Intracellular Glutathione Levels In T Cell Subsets Decrease in HIV-Infected Individuals.
Journal of AIDS Research and Human Retroviruses (1992), Vol. 8, No. 2:305

Y. Suzuki, T. Tanigaki, D. Heimer, W. Wang, W.G.Ross, H.H. Sussman, T.A. Ruffin. Polyethylene Glycol-Conjugated Superoxide Dismutase Attenuates Septic Lung Injury in Guinea Pigs.
American Review of Respiratory Diseases (1992), Vol. 145 (2): 388-392.

T. Tanigaki, Y. Suzuki, D. Heimer, H.H. Sussman, W.G. Ross, T.A,Ruffin. Attenuation of Acute Lung Injury and Oxygen Free Radical Damage by the 21-Aminosteroid Lazaroid U78518F.
Journal of Applied Physiology (1993), 74(5): 2155-2160

L. Robellato, U. Gross, P. Cunningham, R. Riley, W. Ross, R. Knowles and J. Thomas. Treatment of Rhesus Monkey Allograft Recipients With A Combination Of Anti-T-Cell Monoclonal Antibodies.
Transplantation Proceedings (1993) 25 (1 pt 1): 598-9.

R. Riley, D. Taylor, J. Thomas, U. Gross and W. Ross. Histocompatibility Testing For The Sensitized Allograft Recipient.
Immunopathology, (1994), Vol. 16, Number 3.

Y. Suzuki, T. Tanigaki, D. Hovmeir, W. Wang, W. Ross, G. Murphy, A. Sakai, H. Sussman, T. Vu and T. Raffin. TGF-beta1 Causes Increased EndothelialICAM-1 Expression and Lung Injury.
Journal of Applied Physiology (1994), vol. 177 (3): 1281-1287.

T. Tanigaki, Y. Suzuki, D. Heimer, W. Wang, H.H. Sussman, W.G. Ross, G. Murphy and T.A, Ruffin. The Protein Kinase C Inhibitor, H-7, Induces Acute Lung Injury in Guinea Pigs.
Critical Care Medicine (1994), vol. 22 (7): 1167-1173.

Clinical Applications Of Flow Cytometry,
Editors: R. Riley, E. Mahin and W.Ross, Igaku-Shoin Publishers,
New York and Tokyo, 1993. ISBN: 0-89640-200-2

M. Kosiewicz, M. Worthington, W. Ross, J. Matriano, F. Cominelli and A Krishan. FAS-L- expressing APC's can downregulate a soluble protein antigen-specific T-cell respsonse in vivo.
Experimental Biology 99: April 1999

M. Gray, W. Ross, K. Kim and E. Hewlett. Characterization of Binding of Adenylate Toxin to Target Cells by Flow Cytometry.
Infection and Immunity, Sept. 1999, pp 4393-439

C Slingluff, G Yamshckikov, P Neese, H Galavotti, S Eastham, V Engelhard, D Kittlesen, D Deacon, S Hibits, W Grosh, G Petroni, R Cohen, C Wiernasz and W Ross. Phase I Trial of a melanoma vaccine gp10280-288 peptide and tetanus helper peptide in adjuvant: Immunologic and clinical outcomes.
Clinical Cancer Research, March 2001, Vol7, pp 3012-3024.

G Yamshchikov, L Thompson, WG Ross, H Galavotti, W Aquila, D Deacon, J Caldwell, KW Patterson, DF Hunt, CL Slingluff Jr. Analysis of a natural immune response against tumor antigens in a melanoma survivor: lessons applicable to clinical trial evaluations.
Clin Cancer Res. 2001 Mar;7(3 Suppl):909s-916s

M Kosiewicz, A Krishan, M Worrthington and W Ross. B Cells engineered to express FASL suppressed pre-primed antigen-specific T cell responses In Vivo.
Eur Journal of Immunology, June 2002, Vol 6, pp 1679-1687.

Bamias G., M Marini, C Moskaluk, M Odashima, W Ross, J Rivera-Nieves and F Cominelli. Down-regulation of intestinal lymphocyte activation and Th1 cytokine production by antibiotic therapy in a murine model of Crohn’s disease.
Journal of Immunology, Nov 1. 2003, 169, pp 5308014.

Bamias G, Martin C 3rd, Marini M, Hoang S, Mishina M, Ross WG, Sachedina MA, Friel CM, Mize J, Bickston SJ, Pizarro TT, Wei P, Cominelli F. Expression, localization, and functional activity of TL1A, a novel Th1-polarizing cytokine in inflammatory bowel disease.
Journal of Immunology. 2003 Nov 1;171(9):4868-74.

Marini M, Bamias G, Rivera-Nieves J, Moskaluk CA, Hoang SB, Ross WG, Pizarro TT, Cominelli F.TNF-alpha neutralization ameliorates the severity of murine Crohn's-like ileitis by abrogation of intestinal epithelial cell apoptosis.
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8366-71. Epub 2003 Jun

Sullivan GW, Lee DD, Ross WG, DiVietro JA, Lappas CM, Lawrence MB, Linden J. Activation of A2A adenosine receptors inhibits expression of alpha 4/beta 1 integrin (very late antigen-4) on stimulated human neutrophils.
J Leukoc Biol. 2004 Jan;75(1):127-34. Epub 2003 Oct 02.

Olson TS, Bamias G, Naganuma M, Rivera-Nieves J, Burcin TL, Ross W, Morris MA, Pizarro TT, Ernst PB, Cominelli F, Ley K.Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease.
J Clin Invest. 2004 Aug;114(3):389-98.

Bamias G, Martin C, Mishina M, Ross WG, Rivera-Nieves J, Marini M, Cominelli F.
Proinflammatory effects of TH2 cytokines in a murine model of chronic small intestinal inflammation.
Gastroenterology. 2005 Mar;128(3):654-66.

Yamshchikov GV, Mullins DW, Chang CC, Ogino T, Thompson L, Presley J,
Galavotti H, Aquila W, Deacon D, Ross W, Patterson JW, Engelhard VH, Ferrone S, Slingluff CL Jr. Sequential immune escape and shifting of T cell responses in a long-term survivor of melanoma.
J Immunol. 2005 Jun 1;174(11):6863-71.

Bamias G, Mishina M, Nyce M, Ross WG, Kollias G, Rivera-Nieves J, Pizarro TT, Cominelli F. Role of TL1A and its receptor DR3 in two models of chronic murine ileitis.

Proc Natl Acad Sci U S A. 2006 May 30;103(22):8441-6. Epub 2006 May 12

Christensen C, Eschenroeder A, Ross WG, Rie A, Watanabe-Fukunaga, Fukunaga R,

Thomas W. Sturgill. Loss of MNK function sensitizes fibroblasts to serum-withdrawal induced apoptosis
Genes to Cells (2007) 12 (10), 1133–1140.

Instruments Utilized

FACS II, FACS 440, FACStar Plus, FACS Vantage SE
FACS Analyzer, FACScan, FACS Calibur, FACS Aria

Amnis Imagestream

Objectives

It is my objective to obtain employment with an institution that is well equipped with flow cytometric technologies and has a need for advanced flow cytometric data acquisition and analysis. My expertise in flow cytometric techniques and instrumentation allows me to successfully complete both standard and novel flow cytometry applications dealing with cell sorting, surface markers, cytokine measurements, intracellular staining, multi-color staining and complex analysis. I have studied with some of the pioneers in the field of flow cytometry including Sue Sharrow at the NIH as well as Len and Lee Herzenberg at Stanford University and Becton Dickinson design engineers.

References

Peter Ernst, DVM PhD

Professor of Medicine

Gastroenterology and Hepatology

University of Virginia

PBE3E@Virginia.edu

434 924-1944 office

Brian Reuter, PhD

Research Assistant Professor

Professor of Medicine

Gastroenterology and Hepatology

University of Virginia