The problem with compensation is that it needs to be performed on linear data, not logarithmic data. Thus,
either the entire electronics
must be built in linear electronics, which
requires at least 16 bit A-D converters, or a supplementary system must be inserted between the preamp
and the display.
We need the dynamic range for immunologic type markers, but we cant calculate the compensation easily using log
amps - certainly not without
complex math.
Flow cytometers amplify signals to values ranging
between 0-10V before performing a
digital conversion.
Assuming this, with 4 decades and a maximum signal of 10
V we have: