•Scatter
- Rayleigh
Scatter -
directly proportional to property of the scattering molecule called molecular polarizability
(ie dipole formation), inversely proportional to the fourth power of the wavelength of the incident
light (blue light has highest scatter - thus blue sky!)
•Scatter
- Raman Scatter -
(p 93 3rd ed) molecules undergo vibrational transitions at the same time as scatter occurs- if is
transition to higher level is known as Stoke's Raman emission. Normally 1/1000th intensity of Rayleigh
Scatter, but is significantly increased when using lasers for excitation..
Raman emission of water at 488 nm excitation is around 570-590 nm.
•Polarizations
- E vectors -
larger changes in E vectors not incident light plane; Mie scattering - increased scatter in the
forward angle for larger particles (1/4 wavelength to tens of wavelength). (p89, 3rd ed)
•Incident
light, reflected light, transmitted light, refractive index - note the angle
of incidence = angle of reflection regardless of the material of surface. tt transmission angle depends upon the composition of material
according to Snell's law of refraction n1 sin Ti =n2 sin Tt
•n1, n2 are the refractive
indices respectively through which the incident beam passes (air = 1 essentially)
•
•Brewster's
Angle, chromatic
aberration, filters, interference, band pass, dichroic, absorption, laser blocker.
•Fluorescence
lifetime, polarization, fluidity, anisotrophy, resonance energy transfer, quenching, bleaching (p82 3rd ed)