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©1990-2002 J.Paul Robinson, Purdue University
Numerical Aperture
•For a narrow light beam (i.e. closed illumination aperture diaphragm) the finest resolution is (at the brightest point of the visible spectrum i.e. 530 nm)…(closed condenser).
NA
 2 x NA

.00053
2 x 1.00
= 0.265 m
.00053
1.00
= 0.53 m
•
•With a cone of light filling the entire aperture the theoretical resolution is…(fully open condenser)..

=
=