© 2002 J.Paul Robinson, Purdue University
Quenching, Bleaching & Saturation
•Quenching is when excited molecules relax to ground stat5es via nonradiative pathways avoiding fluorescence emission (vibration, collision, intersystem
crossing)
•Molecular oxygen quenches by increasing the probability of intersystem crossing
•Polar solvents such as water generally quench
fluorescence by orienting
around the exited state dipoles
•
Shapiro p 90