•A molecule may undergo a vibrational transition (not an electronic shift) at exactly the same time as scattering occurs
•This results in a photon emission of a photon differing in energy from the energy of the incident photon by the amount of the above energy - this is Raman scattering.
•The dominant effect in flow cytometry is the stretch of the O-H bonds of water. At 488 nm excitation this
would give emission at 575-595 nm