© 2002 J.Paul
Robinson, Purdue University BMS 631- Flow Cytometry lecture002.ppt
Page 27
Rayleigh Scatter
•Molecules and very small particles do not absorb, but scatter light in the visible region (same freq as excitation)
•Rayleigh
scattering is directly proportional to the electric dipole and inversely proportional to the 4th power of the wavelength of the incident light
the sky looks blue
because the gas molecules scatter more
light at shorter
(blue) rather than longer wavelengths (red)
