© 2002 J.Paul Robinson, Purdue University BMS 631- Flow Cytometry lecture002.ppt
Page 25
Interference
Constructive
Interference
Destructive
Interference
A
B
C
D
A+B
C+D
Text Box: Amplitude
Amplitude
0o 
90o 
180o 
270o 
360o 
Wavelength
Figure modified from Shapiro “Practical Flow Cytometry” Wiley-Liss, p79
Here we have a phase difference of 180o (2p radians) so the waves cancel each other out
The frequency does not change, but the amplitude is doubled