© 2002 J.Paul Robinson, Purdue University BMS 631- Flow Cytometry lecture002.ppt
Page 21
Photons and Quantum Theory
•Photons
–particles  have no rest mass - composed of pure electromagnetic energy - the absorption and emission of photons by atoms and molecules is the only mechanism for atoms and molecules can gain or lose energy
•Quantum mechanics
–absorption and emission are quantized - i.e. discrete process of gaining or losing energy in strict units of energy - i.e. photons of the same energy (multiple units are referred to as electromagnetic radiation)
•Energy  of a photon
–can be computed from its frequency (n)
– in hertz (Hz) or its wavelength (l) in meters from
–
»E=hn and E=hc/l
l = wavelength
h = Planck’s constant
(6.63 x 10-34 joule-seconds
c = speed of light (3x108 m/s)
3rd Ed Shapiro p 76