© 2002 J.Paul
Robinson, Purdue University BMS 631- Flow Cytometry lecture002.ppt
Page 11
Biophysics -Ortho
•Ortho Diagnostics (Johnson and Johnson) purchased
Biophysics in 1976 and in
1977 the System 50 Cytofluorograph was developed - this was a droplet sorter, with a flat sided flow cell,
forward and orthogonal scatter,
extinction, 2 fluorescence parameters, multibeam excitation, computer analysis option.
•1979 - NIH scientists had added a krypton laser at 568 nm to excite Texas Red fluorescence at 568 nm and emit at 590-630 nm.
Argon (488 nm FITC was
measured simultaneously without signal cross-talk - thus the FACS IV was developed (B-D).
Photo ©2000 – J.P. Robinson
