© 2002 J.Paul
Robinson, Purdue University BMS 631- Flow Cytometry lecture002.ppt
Page 9
Hemalog D
Technicon - Hemalog D - 1974 - first commercial differential flow
cytometer - light scatter and absorption at different
wavelengths - chromogenic enzyme substrates were used to identify neutrophils and
eosinophils by peroxidase and monocytes by esterase, basophils were identified by the presence of
glycosaminoglycans using Alcian Blue - the excitation for all measurements was a tungsten-halogen
lamp
Insert
photos on page 60
Image from Shapiro “Practical Flow Cytometry”, Wiley-Liss, 1995
