© 2002 J.Paul Robinson, Purdue University BMS 631- Flow Cytometry lecture002.ppt
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Hemalog D
Technicon - Hemalog D - 1974 - first commercial differential flow cytometer -  light scatter and absorption at different wavelengths - chromogenic enzyme substrates were used to identify neutrophils and eosinophils by peroxidase and monocytes by esterase, basophils were identified by the presence of glycosaminoglycans using Alcian Blue - the excitation for all measurements was a tungsten-halogen lamp
Insert photos on page 60
Image from Shapiro “Practical Flow Cytometry”, Wiley-Liss, 1995