© 2002 J.Paul
Robinson, Purdue University BMS 631- Flow Cytometry lecture002.ppt
Herzenberg
& Becton Dickinson
Herzenberg -1972 - Argon laser
flow sorter - placed an argon laser onto their sorter and successfully did high speed
sorting - Coined the term Fluorescence Activated Cell
Sorting (FACS) This instrument could
detect weak fluorescence with rhodamine and fluorescein tagged antibodies. A commercial
version was distributed by B-D in 1974 and could collect forward scatter and fluorescence
above 530 nm.
Photo ©2000 – J.P. Robinson