© 2002 J.Paul Robinson, Purdue University BMS 631- Flow Cytometry lecture002.ppt
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Herzenberg & Becton Dickinson
Herzenberg -1972 -  Argon laser flow sorter - placed an argon laser onto their sorter and successfully did high speed sorting - Coined the term Fluorescence Activated Cell Sorting (FACS) This instrument could detect weak fluorescence with rhodamine and fluorescein tagged antibodies. A commercial version was distributed by B-D in 1974 and could collect forward scatter and fluorescence above 530 nm.
Photo ©2000 – J.P. Robinson