© 2002 J.Paul
Robinson, Purdue University BMS 631- Flow Cytometry lecture002.ppt
Page 14
Concepts
Scatter: Size,
shape, granularity, polarized scatter
(birefringence)
Fluorescence:
Intrinsic: Endogenous pyridines and flavins
Extrinsic:
All other fluorescence profiles
Absorption: Loss of
light (blocked)
Time: Useful for
kinetics, QC
Count: Always part of
any collection
