ŠJ.Paul Robinson, Purdue University  BMS 631 - LECTURE1.PPT
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Historical Overview
V. Early flow systems
Hallermann et al, Kosenow - 1964 -  AO staining of leukocytes - was able to use fluorescence (in a flow based system)  to select leukocytes from red cells despite a low ratio (1/1000) because they took up lots of AO - also claimed to be able to discriminate between monocytes and PMN