©J.Paul Robinson, Purdue University  BMS 631 - LECTURE1.PPT
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Stains...
Up to 1850’s - only natural stains were available such as Saffron (which was what Leeuwenhoek used to stain muscle cells)
Ehrlich -  used acidic and basic dyes to identify acidophilic, eosinophilic, basophilic and neutrophilic leukocytes 1880’s to study the dynamics of ocular fluids- used fluorescein
Fluorescence UV Microscope - August Köhler - 1904
Pappenheim & Unna  (early 1900’s) - combined methyl green and pyronin to stain nuclei green and cytoplasm red
Robert Feulgen (1925) - demonstrated that DNA was present in both animal and plant cell nuclei - developed a stoichiometric procedure for staining DNA involving a derivatizing dye, (fuchsin) to a Schiff base