Stains...
Up
to 1850’s - only
natural stains were available such as Saffron (which was what Leeuwenhoek used to
stain muscle cells)
Ehrlich - used acidic and basic dyes to identify acidophilic,
eosinophilic, basophilic and neutrophilic leukocytes 1880’s to study the dynamics of
ocular fluids- used fluorescein
Fluorescence UV Microscope - August Köhler
- 1904
Pappenheim & Unna (early 1900’s) - combined methyl green and
pyronin to stain nuclei green and cytoplasm red
Robert Feulgen
(1925) - demonstrated
that DNA was present in both animal and plant cell nuclei - developed a stoichiometric procedure for staining DNA involving a derivatizing
dye, (fuchsin) to a Schiff base
