Lecture 2
Clinical Applications of Flow Cytometry
Primary areas
Purdue University Cancer Center & Purdue University Cytometry Laboratories
Brief Introduction to Flow Cytometry
PPT Slide
A Histogram (a frequency distribution graph)
DNA Probes
DNA/RNA Probes
The Cell Cycle
Definitions & Terms
A DNA histogram
A typical DNA Histogram
Multiparameter gating
Measurement of Apoptosis
Detection Methods for Apoptotis
Flow Cytometry of Apoptotic Cells
Labeling Strand Breaks with dUTP [Fluorescein-deoxyuridine triphosphate (dUTP)]
Nuclear Antigens
Nuclear antigens
Differential Inflammatory Cell Count
Simultaneous UV & Visible Light
Hoechst & PI Fluorescence
Boar Sperm
Human Sperm
Human Sperm - PI - Sybr-Green I
Microbiology
Uptake of rhodamine 123 by M.luteus
Light Scatter of Bacterial Spores
Microbial Identification Using Antibodies
Phenotyping - Immunophenotyping
Immunofluorescence staining
Direct staining
Indirect staining
Avidin-Biotin method I
Three Color Lymphocyte Patterns
From Duque et al, Clin.Immunol.News.
Cellular Function
Conclusions
Thank you for your attention
Email: jpr@flowcyt.cyto.purdue.edu
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