Table of Contents
Biomedical technologies for blood cell measurements
What can Flow Cytometry Do?
PPT Slide
Papanicolaou
Gucker - 1947
P.J. Crossland-Taylor
Wallace Coulter
The Coulter Principle
Wallace Coulter - Coulter orifice - 1948-1956
Instrument Components
What are the principles?
Richard Sweet
Mack Fulwyler
The mysterous red cell problem
PPT Slide
Kamentsky
PPT Slide
PPT Slide
PPT Slide
Hydrodynamics and Fluid Systems
Fluidics
Fluidics - Laminar Flow
Fluidics
Fluidics Systems
Syringe systems
Fluidics - Particle Orientation and Deformation
Closed flow cells
Fluidics - Flow Chambers
Flow chamber blockage
The Elements of Flow Sorting
PPT Slide
Droplet formation
Droplet break off
Laser power
Light Scatter
Optics for forward scatter
Brewster’s Angle
Brewster’s Angle
Fluorescence
Fluorescence
PPT Slide
Fluorescence
Absorption
Mercury Arc Lamps
Arc Lamp Excitation Spectra
Laser Power & Noise Light Amplification by Stimulated Emission of Radiation
Lasers
Goals of Light Collection
Optical Collection systems
Interference in Thin Films
Interference and Diffraction: Gratings
Interference filters
Optical Filters
PPT Slide
Transmission determination
PPT Slide
PMT
Signal Detection - PMTs
Diode Vs PMT
Review of Electronics
Linear and Log circuits
Why use linear amps?
How many bits?
Ideal log amp
Log amps & dynamic range
Data Acquisition
Data Acquisition - Listmode
Data Presentation Formats
PPT Slide
Data Analysis
Coefficient of Variation
Precision - C.V.
One parameter frequency histogram
PPT Slide
Kolmogorov-Smirnov K-S Test
Histogram Analysis Integration
PPT Slide
Histogram Overlays
Density Dot Plot Contour Plot
PPT Slide
Isometric Plot 3 Parameter
Multi-color studies generate a lot of data
From Duque et al, Clin.Immunol.News.
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