Labelling Strand Breaks with dUTP [Fluorescein-deoxyuridine triphosphate (dUTP)]

Green Fluorescence is Tdt and biotin-dUTP followed by fluorescein-streptavidin

Red fluorescence is DNA counter-stained with 20µg/ml PI

PI-Red Fluorescence

Green Fluorescence

Green Fluorescence

Side Scatter

Forward Scatter

Green:

apoptotic cells

Red:

normal cells

R2: Apoptotic Cells

R1:

Normal

Cells

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