Methods in Cell Biology: Cytometry, 3rd Edition, Part A, Vol. 63
Zbigniew Darzynkiewicz
Harry A. Crissman
J.Paul Robinson

Academic Press, San Diego, October, 2000

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Table of Contents

CYTOMETRY (Third Edition), Part A, Vol. 63

Zbigniew Darzynkiewicz, Harry A. Crissman, and J. Paul Robinson (Eds)

Chapter 1

Myron R. Melamed -- Cytometry. Historical Perspective

Chapter 2

Alice L. Givan -- Principles of Flow Cytometry: an Overview

Chapter 3

Louis A. Kamentsky -- Laser Scanning Cytometry

Chapter 4

J. Paul Robinson -- Principles of Confocal Microscopy

Chapter 5

Howard M. Shapiro

-- Optical Measurements in Cytometry: Light Scattering, Extinction, Absorption and Fluorescence.

Chapter 6

Harry A. Crissman and John A. Stainkamp -- Flow Cytometric Fluorescence Lifetime Measurements

Chapter 7

Howard M. Shapiro -- Principles of Data Acquisition and Display

Chapter 8

Larry Seamer and Larry A. Sklar -- Time as a Flow Cytometric Parameter

Chapter 9

Kevin L. Holmes and Larry M. Lantz -- Protein Labeling with Fluorescent Probes



Chapter 10

J. Philip McCoy, Jr -- Preparation of Cells from Blood

Chapter 11

Carleton C. Stewart and Sigrid J. Stewart -- Cell Preparation for Identification of Leukocytes

Chapter 12

Steven K. Koester and Wade E Bolton -- Strategies for Cell Permeabilization and Fixation in Detecting Surface and Intracellular Antigens


Chapter 13

James W. Jacobberger -- Stoichiometry of Immunochemical Staining Reactions and Flow Cytometry of Intracellular Antigens

Chapter 14

Robert A. Hoffman -- Standardization and Quantitation in Flow Cytometry



Chapter 15

Gloria Juan, Frank Traganos and Zbigniew Darzynkiewicz -- Identification of Mitotic Cells by Flow Cytometry

Chapter 16

Nicholas H.A. Terry and R. Allen White -- Cell Cycle Kinetics Estimated by Analysis of BrdUrd Incorporation

Chapter 17

A. Bruce Lyons, Jhagvaral Hasbold and Philip D. Hodgkin -- Flow Cytometric Analysis of Cell Division History Using Dilution of CFSE, as Stably Integrated Fluorescent Probe

Chapter 18

Elmar Endl, Christiane Hollman and Johannes Gerdes -- Antibodies against the Ki-67 Protein: Assessment of the Growth Fraction and Tools for Cell Cycle Analysis

Chapter 19

Jorgen K. Larsen, Göran Landberg and Göran Roos -- Detection of Proliferating Cell Nuclear Antigen (PCNA)

Chapter 20

Andrea Fattorossi, Alessandra Battaglia and Cristiano Ferlini -- Lymphocyte Activation Associated Antigens


Chapter 21

Andrea Cossarizza and Stefano Salvioli -- Analysis of Mitochondria during Cell Death

Chapter 22

Steven K. Koester and Wade E. Bolton -- Cytometry of Caspases

Chapter 23

Iona E. Weir -- Analysis of Apoptosis in Plant Cells

Chapter 24

Zbigniew Darzynkiewicz, Elzbieta Bedner and Frank Traganos -- Analysis of Apoptosis: Common Pitfalls and Misconceptions


Chapter 25

Nicole Dodger Zantek and Michael S. Kinch -- Analysis of Cell Migration

Chapter 26

Sherry L. Voytik-Harbin -- 3D Extracellular Matrix Substrates for Cell Culture

Chapter 27

5. S.L. Voytik-Harbin, B. Rajwa and J.P. Robinson -- 3D imaging of Extracellular Matrix and Extracellular Matrix-Cell Interactions

Chapter 28

Michael S. Kinch -- Cytometric Analysis of Cell Contact and Adhesion

Chapter 29

Emma T. Bowden, Peter J. Coopman and Susette C. Mueller -- Invadopodia: Unique Methods for Measurement of Extracellular Matrix Degradation in vitro


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