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Spectral Flow Cytometry - the real story

Before Spectral Flow Cytometry: For many years we have focused on polychromatic flow cytometry as being the fundamental tennat of flow cytometry. We moved from 1 or 2 colors, to three colors to 8, 12, 18, 27 and more. But the technology never really changed. All we did was add more PMTs and select more lasers. While this works pretty well, there are many complications that make doing high color polychromatic flow cytometry very difficult. There may well be a better way!

 

Spectral flow cytometry is taking off. We would like to set the record straight. The core development of spectral flow cytometry was first presented by the Purdue group at the 2004 ISAC meeting in Montpellier and published by our group in a biophotoncis International paper here in 2004. A paper was published in SPIE in 2005. Purdue submitted a provisional patent on April 8, 2004 and the patent US7280204 was issued on Oct 9, 2007. Another paper on our work was published in 2011. This patent covers all applications and use of spectral flow cytometry. The patent was licensed by Sony Life Sciences and is the basis of the technology used in the Sony Spectral flow cytometer. For some reason SONY has chosen never to publically acknowledge that their spectral flow cytometer is based on a licence to the Purdue technology.

 

The issued patent US7280204 covers both the hardware to achieve spectral flow cytometry AND the analytical component. So the use of spectral unmixing for example, to identify a spectific probe and define a phenotype is covered under the patent. The ONLY company that has licenced the patent is SONY - all other companies selling flow cytometers for using spectral analysis are infringing the Purdue patent. 

 

 

 

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