Local, Three-dimensional Strain Measurements within Largely-Deformed Extracellular Matrix Constructs

Supplementary Information

Blayne A. Roeder (1,2), Klod Kokini (1,2), J. Paul Robinson (1,3), Sherry L. Voytik-Harbin (1,3)

1. Department of Biomedical Engineering, Purdue University, West Lafayette, IN

2. School of Mechanical Engineering, Purdue University, West Lafayette, IN

3. Department of Basic Medical Sciences, Purdue University, West Lafayette, IN

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2-3 plane
1-2 plane	1-3 plane

Video 1 (2.5-13.5Mb): Time-lapse confocal reflection images demonstrating load-induced micro-structural deformation of collagen fibrils within a collagen ECM as recorded by the mechanical loading-confocal microscopy set-up. A confocal microscope adapted with a mechanical loading instrument was used to visualize changes in the 3D micro-structure of the collagen ECM during loading from 0-30% (0.3 mm/mm) strain. Upon loading, collagen fibrils not only become aligned in the loading direction (1-direction) but also they become more compact.

Video 2 (31.5Mb): Movie illustrating the 3D response of a 1 mg/ml collagen ECM to tensile mechanical strain in the direction indicated by the arrows. Maximum principle strain is plotted on the contour.